Journal: PLoS Biology

Article Title: Distinct Genomic Integration of MLV and SIV Vectors in Primate Hematopoietic Stem and Progenitor Cells

doi: 10.1371/journal.pbio.0020423

Figure Lengend Snippet: Shown are integrations that landed within RefSeq gene introns (arrows) in comparison to in silico-generated integration sites (bars). Black indicates MLV and gray indicates SIV. * p < 0.0001 by a Chi 2 test.

Article Snippet: We correlated the 760 integration sites (432 for MLV and 328 for SIV) that unequivocally mapped to a unique position in the genome to the locations of annotated genes, using the UCSC Genome Browser Reference Sequence (RefSeq) Genes track, which displays the positions of National Center for Biotechnology Information mRNA Reference Sequences ( ).

Techniques: Comparison, In Silico, Generated

Journal: PLoS Biology

Article Title: Distinct Genomic Integration of MLV and SIV Vectors in Primate Hematopoietic Stem and Progenitor Cells

doi: 10.1371/journal.pbio.0020423

Figure Lengend Snippet: (A) Distribution of MLV and SIV integration sites relative to gene density within a 1-Mbp window compared to in silico-generated random integration sites. Each bar corresponds to the percentage of integration sites within the corresponding gene density region. (B) Location of MLV and SIV integration sites and gene density on human Chromosome 6. MLV and SIV integrations were aligned to Chromosome 6 (obtained from the UCSC custom annotation track feature) and shown in relation to RefSeq gene density (blue). 73% of the SIV integration events are within the 20-Mbp unique ridge of Chromosome 6, compared to 29% for MLV. Distance between thick black bars is 20 Mbp; centromere is represented by the black circle.

Article Snippet: We correlated the 760 integration sites (432 for MLV and 328 for SIV) that unequivocally mapped to a unique position in the genome to the locations of annotated genes, using the UCSC Genome Browser Reference Sequence (RefSeq) Genes track, which displays the positions of National Center for Biotechnology Information mRNA Reference Sequences ( ).

Techniques: In Silico, Generated

Journal: Canadian Journal of Veterinary Research

Article Title: ACTN3 gene variants as potential phenotype and performance biomarkers in Brazilian sport horses training for eventing in a tropical climate

doi:

Figure Lengend Snippet: Significant correlation (P = 0.017) between the mean thickness (± standard deviation) of the subcutaneous fat layer in the region of the gluteus medius, as measured by ultrasonography, and the ACTN3 sequence variant c.1681–129delA in 17 Brazilian sport horses training for events in a tropical climate.

Article Snippet: The equine ACTN3 reference genomic sequence used in the analysis was retrieved from GenBank [National Center for Biotechnology Information (NCBI), Bethesda, Maryland, USA] with the accession number NC_00009155.2.

Techniques: Standard Deviation, Sequencing, Variant Assay